Megabase-sized DNA is crucial to modern genomics research of all organisms. Among the preparation methods developed, the nuclei method is the simplest and most widely used for preparing high-quality megabase-sized DNA from divergent organisms. In this method, nuclei are first isolated by physically grinding the...
Nature Protocols 7, 467 (2012). doi:10.1038/nprot.2011.455
Authors: Meiping Zhang, Yang Zhang, Chantel F Scheuring, Cheng-Cang Wu, Jennifer J Dong & Hong-Bin Zhang
(Via Nature Protocols.)