Cambridge website for Synthetic Biology Resources

synbio logo2_100a

Compiled by Jim Haseloff at the University of Cambridge.

This site contains details of recent papers and activity in Synthetic Biology, with particular emphasis on: (i) development of standards in biology and DNA parts, (ii) microbial and (iii) plant systems, (iv) hardware for scientific computing and instrumentation, (v) tools for scientific productivity and (vi) collected miscellany.

The site also contains details of Synthetic Biology research and teaching at the University of Cambridge, including the annual iGEM team run by Jim Ajioka, Jim Haseloff and Gos Micklem in Cambridge.

 

www.synbio.org.uk

SpannerPlantLogo70 logoplate70  

SynBio calendar

  • 04 Jun

    Fourth International Workshop on Bio-Design Automation (IWBDA)

    The Fourth International Workshop on Bio-Design Automation (IWBDA) at DAC will bring together researchers from the synthetic biology, systems biology, and design automation communities....

  • 06 Jun

    New Phytologist Workshop: Synthetic Biology

    The overall goal for the workshop is to bring together scientists working in the highly interdisciplinary field of synthetic biology to present cutting-edge research aligned with three...

  • 20 Jun

    GCAT Synthetic Biology Workshop

    GCAT is pleased to announce a synthetic biology faculty workshop for the summer of 2012 (June 20-22) hosted by HHMI’s Science Education Alliance (SEA). The goal of this workshop...

  • 25 Jun

    enGENEious: Evolving Life for Future Technologies

    A student and post-doc organised conference: they have invited the world's leading scientists to highlight the recent advances in microbial engineering, along with discussing the challenges...

  • 30 Jul

    BioBuilding 2012 workshop

    A week long, professional development class will prepare educators to bring biological engineering and synthetic biology into their classrooms and laboratories. The workshop will include...

  • 24 Sep

    Synthetic Biology meeting in Cambridge, UK

  • 02 Nov

    iGEM2012 World Championship Jamboree

    Finals for the international Genetically Engineered Machine Competition.

  • 26 Nov

    CSH Asia Conference: Synthetic Biology

    The 2nd CSH Asia Synthetic Biology meeting will be held at the Suzhou Dushu Lake Conference Center in Suzhou, China, located approximately 60 miles west of Shanghai.

  • 09 Jun

    Gordon Conference: Synthetic Biology

    (Re-)constructing and Re-programming Life

04 Jun - 09 Jun
20 Jun - 27 Jun
30 Jul - 28 Sep
02 Nov - 01 Dec
09 Jun - 15 Jun

SynBio Google newsfeed

Loading...
Synbio news:
22 Jan 2012

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.: "Publication Date: 2012 Jan 18 PMID: 22259038
Authors: Heap, J. T. - Ehsaan, M. - Cooksley, C. M. - Ng, Y. K. - Cartman, S. T. - Winzer, K. - Minton, N. P.
Journal: Nucleic Acids Res

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of heterologous counter-selection markers, here we explore novel DNA integration strategies that do not employ them, and instead exploit (i) activation or inactivation of genes leading to a selectable phenotype, and (ii) asymmetrical regions of homology to control the order of recombination events. We focus here on the industrial biofuel-producing bacterium Clostridium acetobutylicum, which previously lacked robust integration tools, but the approach we have developed is broadly applicable. Large sequences can be delivered in a series of steps, as we demonstrate by inserting the chromosome of phage lambda (minus a region apparently unstable in Escherichia coli in our cloning context) into the chromosome of C. acetobutylicum in three steps. This work should open the way to reliable integration of DNA including large synthetic constructs in diverse microorganisms.

post to: CiteULike"

(Via Nucleic Acids Research.)

Site features

RSS newsfeeds

PhD Studies in Cambridge

The Board of Graduate Studies manages admission of the University's graduate students. Prospective students should start here - for an introduction to the University of Cambridge, the courses we offer, how to apply for postgraduate study, how your application will be processed, and immigration and other important information.

Click here for more information about Cambridge

 

Weather in Cambridge

22°
°F°C
Cambridge, UK
Clear
Humidity: 69%
Wind: N at 14 mph
Tue
Mostly Sunny
8 | 22
Wed
Mostly Sunny
12 | 25
Thu
Mostly Sunny
11 | 26
Fri
Clear
8 | 17

Instrumentation

DNA parts

Plant systems

Productivity