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- An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli.
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An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli.
An automated home-built low-cost fermenter for Escherichia coli.
We have developed an automated fermentation system for cost-efficient upscaling of protein expression in bacteria. The system, built for use by nonbiotechnologists, can be assembled mostly from standard laboratory equipment and allows a largely unattended growth of bacteria to OD 25 (at 600 nm) in a 12 L vessel. The typical yield of 250-350 g of wet weight cell pellet per run, which is equivalent to the biomass obtained from 250 shake flask cultures containing 400 mL Luria-Broth medium each, facilitates the production of large amounts of purified recombinant protein without the laborious need for optimization of expression and purification conditions.
Publication Date: 2008 Aug PMID: 18687068
Authors: Riek, U. - Tuerk, R. - Wallimann, T. - Schlattner, U. - Neumann, D.
Journal: Biotechniques
