The iGEM2010 Jamboree is here again, running Nov 6th-8th. The Cambridge team presented the E.glowli story, showing a set of BioBricks to allow generation of bioluminescence in a wide range of colours which have applications both as reporters for biosensors and as natural light sources.
A number of strategies were used to extend the use of firefly luciferase: (i) codon optimisation for increased light output, (ii) use of a luciferin regenerating enzyme, and (iii) mutagenesis to create a number of different colours.
Light producing systems from Vibrio fischeri were also explored. The team created the first BioBrick to emit light in normal E. coli strains without the addition of any external substrate.
The team made extensive use of Gibson Assembly to manufacture these parts, and have submitted an RFC to the BioBricks Foundation to help promote use of this technique.
In addition, new software tools were built. (i) Gibthon Construct Designer allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for Gibson Assembly. (ii) BioBrick → GenBank allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software. (iii) The Ligation Calculator is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
Finally, the E.glometer is a cheap, easily built, piece of electronics for measuring bioluminescence. It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in quantitative biosensors.