Newsflash:

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The iGEM2010 Jamboree is here again, running Nov 6th-8th. The Cambridge team presented the E.glowli story, showing a set of BioBricks to allow generation of bioluminescence in a wide range of colours which have applications both as reporters for biosensors and as natural light sources. 

A number of strategies were used to extend the use of firefly luciferase: (i) codon optimisation for increased light output, (ii) use of a luciferin regenerating enzyme, and (iii) mutagenesis to create a number of different colours.

Light producing systems from Vibrio fischeri were also explored. The team created the first BioBrick to emit light in normal E. coli strains without the addition of any external substrate.

The team made extensive use of Gibson Assembly to manufacture these parts, and have submitted an RFC to the BioBricks Foundation to help promote use of this technique.

In addition, new software tools were built. (i) Gibthon Construct Designer allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for Gibson Assembly. (ii) BioBrick → GenBank allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software. (iii) The Ligation Calculator is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.

Finally, the E.glometer is a cheap, easily built, piece of electronics for measuring bioluminescence. It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in quantitative biosensors.

synbio.org.uk

iGEM news

iGEM2010 Jamboree results

  Grand Prize, Winner of the BioBrick Trophy: Slovenia 1st Runner Up: Peking 2nd Runner Up: BCCS-Bristol Finalists: BCCS-Bristol Cambridge Imperial College London Peking Slovenia TUDelft Track Award Winners: Best Food or Energy Project: BCCS-Bristol Best Environment Project: Peking Best Health or Medicine Project: Washington & Freiburg Bioware (Tie) Best Information Processing Project: ETHZ...
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Finding the key - cell biology and science education.

Finding the key - cell biology and science education.: "Publication Date: 2010 Sep 20 PMID: 20863704
Authors: Miller, K. R.
Journal: Trends Cell Biol

No international research community, cell biology included, can exist without an educational community to renew and replenish it. Unfortunately, cell biology researchers frequently regard their work as independent of the process of education and see little reason to reach out to science teachers. For...
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iGEM2010 sponsors

We would like to thank everyone who is helping us out with iGEM 2010.   Sponsors at the University of Cambridge:   The School of Biological Sciences, 
Department of Genetics, 
Department of Plant Sciences, 
Department of Biochemistry, 
Department of Physiology, Neurobiology & Development, 
The School of Technology, 
Department of Engineering, Division of Life Sciences, 
Department of Chemical Engineering and Biotechnology (SynBio2010)   The University of Cambridge iGEM team and organisers...
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SynBio2010 course

Timetable for 2010 Work Groups for SynBio2010 tasks & student photos Synthetic Biology website in Cambridge (www.synbio.org.uk)
Course photographs
Course Assessment Read More...

iGEM: the student synthetic biology experience

iGEM: the student synthetic biology experience by Mun-Keat Looi, Wellcome Trust blog, http://wellcometrust.wordpress.com/2010/04/01/igem-the-student-synthetic-biology-experience/   European teams, including Imperial and Cambridge at the 2009 iGEM jamboree finals at MIT. Making anything work in genetic engineering is difficult in itself, but doing...
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'Building block' biology

The new field of synthetic biology aims to make biology controllable, predictable and designable. Mun-Keat Looi asks if you can really engineer a biological organism and hears how a unique competition for undergraduates is helping the field gather momentum. What if you could engineer an organism to do whatever you want: produce life-saving drugs cheaply, generate energy, or detect and clear waste from a polluted lake? And what if building that organism was like constructing a model using toy bricks or piecing together an electronic circuit? Welcome to the world of synthetic biology. "The...
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