Microbial fermentations and bioconversions play a central role in the production of pharmaceuticals, enzymes and chemicals. To meet the demands of industrial production, it is desirable that microbes maintain a maximized carbon flux towards target metabolites regardless of fluctuations in intracellular or extracellular environments. This requires cellular systems that maintain functional...
Engineering tools for Gram positive bacteria
Gram positive bacteria like Bacillus subtilis provide a number of benefits for synthetic biologists. B. subtilis is non-tranformable and naturally transformable, with an efficient system for homologous transformation. Gram positive bacteria provide the bulk of industrially important species and their architectures allow high level secretion of enzymes and other proteins. The Synthetic Biology group at the University of Cambridge is developing tools for work with B. subtilis. The pages in this section provide access to recent papers and relevant websites in the field.
Microbe news:
- Microbes breathe life into oxygen theory
- [Research highlight] Laws of microbial growth
- 3D imaging of the highly bent architecture of Bdellovibrio bacteriovorus using cryo-electron tomography.
- 90 billion tons of microbial organisms live in the deep biosphere
- A negative feedback loop involving small RNAs accelerates Vibrio cholerae's transition out of quorum-sensing mode.
- A photosensory two-component system regulates bacterial cell attachment
- A Quorum on Bacterial Programmed Cell Death.
- A sensor histidine kinase co-ordinates cell wall architecture with cell division in Bacillus subtilis.
- A sensor histidine kinase co-ordinates cell wall architecture with cell division in Bacillus subtilis.
- A wall of funnels concentrates swimming bacteria.
- A Wall of Funnels Concentrates Swimming Bacteria.
- Active and passive mechanisms of intracellular transport and localization in bacteria.
- Advantages and mechanisms of polarity and cell shape determination in Caulobacter crescentus.
- An engineered microbial platform for direct biofuel production from brown macroalgae.
- Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR.
- Bacillus Genome Stock Center news
- Bacillus protein secretion: an unfolding story.
- Bacillus Standards Working Group Meeting 1
- Bacteria and Algae act as biocatalysts for deep-sea raw material deposition
- Bacteria are able to extend psuedo-legs and walk upright
- Bacteria with a built-in thermometer
- Bacterial cell wall engineering
- Bacterial chemoreceptors: high-performance signaling in networked arrays.
- Bacterial development: Moving in the right direction
- Bacterial morphogenesis: learning how cells make cells.
- Bacterial morphology: why have different shapes?
- Bacterial morphology: why have different shapes?
- Bacterial physiology: Bacillus takes the temperature
- Bacterial secretion: Structural snapshot
- Bacterial-plant communications and soft rot.
- Bet-hedging and epigenetic inheritance in bacterial cell development.
- BioBrick vectors for Bacillus subtilis
- Biogenic Insecticides
- Bottom-up genome assembly using the Bacillus subtilis genome vector
- Bottom-up genome assembly using the Bacillus subtilis genome vector.
- Bottom-up genome assembly using the Bacillus subtilis genome vector.
- Cell shape determination in Escherichia coli.
- Cell shape determination in Escherichia coli.
- Cellular microbiology: All aboard the cell-wall shuttle
- Cellular microbiology: Synchronized switching
- Chemosensory pathways, motility and development in Myxococcus xanthus
- Coding-sequence determinants of gene expression in Escherichia coli
- Colours of microbes
- Contact-dependent signaling in bacterial populations.
- Cooperation and conflict in quorum-sensing bacterial populations
- Coordinating cell cycle events during bacterial growth and division.
- Defying stereotypes: the elusive search for a universal model of LysR-type regulation.
- Determination of bacterial rod shape by a novel cytoskeletal membrane protein
- Diatoms and nanotech
- Direct selection and phage display of a Gram-positive secretome.
- Dissecting the quorum-sensing receptor LuxN.
- DIY molecular genetics supplies
- Dual KaiC-based oscillations constitute the circadian system of cyanobacteria.
- Duplication and segregation of the actin (MreB) cytoskeleton during the prokaryotic cell cycle
- Dynamics in the mixed microbial concourse.
- E. chromi Designer Bacteria Will Color Your Poop According to What Ails You
- Ecology and genomics of Bacillus subtilis.
- Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1.
- Engineering microbial consortia: a new frontier in synthetic biology.
- Engineering the robustness of industrial microbes through synthetic biology.
- Escherichia coli DnaA forms helical structures
- Exosomes - vesicular carriers for intercellular communication.
- Extreme polyploidy in a large bacterium.
- Functional expression in Bacillus subtilis of mammalian NADPH-cytochrome P450 oxidoreductase and its spore-display.
- Genome sequence of the solvent-producing bacterium Clostridium carboxidivorans strain P7T.
- Genomic comparison of ocean microbes reveals East-West divide in populations
- HORTUS is a Crazy Greenhouse Contraption of 300+ Algae-Filled Bags
- How a cyanobacterium tells time.
- Identification of Residues Important for Cleavage of the Extracellular Signaling Peptide, CSF, of B. subtilis From Its' Precursor Protein.
- Imaging mass spectrometry of intraspecies metabolic exchange revealed the cannibalistic factors of Bacillus subtilis.
- In an early branching metazoan, bacterial colonization of the embryo is controlled by maternal antimicrobial peptides.
- Infectious bacteria in your gut create black market for weapons | Not Exactly Rocket Science | Discover Magazine
- Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.
- Inter-kingdom signalling: communication between bacteria and their hosts
- Leonard et al Engineering microbes
- Methods for unveiling cryptic microbial partnerships in nature
- Microbe networking
- Microbes churn out hydrogen at record rate
- Microbial Cell Factories: Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis
- Microbial communities in industrial environment
- Microbial diversity in pitcher plants
- Microbial production of plant benzylisoquinoline alkaloids
- Microbial regulatory and metabolic networks.
- Microbubbles Enable More Efficient Production Of Algae Biofuels
- Millimeter-scale genetic gradients and community-level molecular convergence in a hypersaline microbial mat
- Mimivirus
- MreB: pilot or passenger of cell wall synthesis?
- Multicellular Life Evolves in Laboratory
- NASA Wants to Power Robots With Microbes
- Networked microbes
- New antibiotics
- New fluorescence microscopy methods for microbiology
- New images of marine microbe illuminate carbon and nitrogen fixation
- New tools for discovering and characterizing microbial diversity.
- Nitrogen fixation by marine cyanobacteria.
- Non-signaling properties of quorum sensing molecules
- Noncoding RNA control of the making and breaking of sugars.
- Npro fusion technology to produce proteins with authentic N termini in E. coli
- Oceans' smallest lifeforms
- Overview of cell shape: cytoskeletons shape bacterial cells.
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Prospecting macroalgae (seaweeds) as feedstocks for bioconversion into biofuels and commodity chemical compounds is limited primarily by the availability of tractable microorganisms that can metabolize alginate polysaccharides. Here, we present the discovery of a 36-kilo-base pair DNA fragment from Vibrio splendidus encoding enzymes for alginate transport and metabolism. The genomic integration...
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HORTUS — or Hydro Organisms Responsive To Urban Stimuli — is a crazy new exhibit in London made up of 325 transparent bags of algae hanging from the ceiling via a network of ropes. Long plastic tubes hang from the bags and visitors are instructed to blow into them to feed the algae — which ranges in color from dark green, to pink, to brown — with their breath. Devised by...
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E. chromi is an experimental collaboration between designers and scientists working in the field of synthetic biology. Royal College of Art graduates’ James King and Daisy Ginsberg, together with University of Cambridge’s iGEM 2009 Biology team, are developing a cheap, personalized disease monitoring system that works from the inside out. By color-coding diseases and giving a patient an E....
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Algae biofuel is one of the most promising alternative fuels on the market – so far we’ve seen cars and even planes adapted to run on it. The main drawback thus far has been high production costs and energy usage – until now. Using a new “cost-effective harvesting method” featuring microbubbles, a team from the University of Sheffield believe they have found a way to make algae a more...
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MreB: pilot or passenger of cell wall synthesis?: "Publication Date: 2011 Dec 7 PMID: 22154164 Authors: White, C. L. - Gober, J. W. Journal: Trends Microbiol The discovery that the bacterial cell shape determinant MreB is related to actin spurred new insights into bacterial morphogenesis and development. The trafficking and mechanical roles of the eukaryotic cytoskeleton were hypothesized to...
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Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.: "Publication Date: 2012 Jan 18 PMID: 22259038 Authors: Heap, J. T. - Ehsaan, M. - Cooksley, C. M. - Ng, Y. K. - Cartman, S. T. - Winzer, K. - Minton, N. P. Journal: Nucleic Acids Res Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon...
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Defying stereotypes: the elusive search for a universal model of LysR-type regulation.: "Publication Date: 2012 Feb PMID: 22235937 Authors: Momany, C. - Neidle, E. L. Journal: Mol Microbiol LysR-type transcriptional regulators (LTTRs) compose the largest family of homologous regulators in bacteria. Considering their prevalence, it is not surprising that LTTRs control diverse metabolic functions....
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Rewiring two-component signal transduction with small RNAs.: "Publication Date: 2011 Dec 23 PMID: 22197250 Authors: Gopel, Y. - Gorke, B. Journal: Curr Opin Microbiol Bacterial two-component systems (TCSs) and small regulatory RNAs (sRNAs) form densely interconnected networks that integrate and transduce information from the environment into fine-tuned changes of gene expression. Many TCSs...
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Systems and synthetic metabolic engineering for amino acid production - the heartbeat of industrial strain development.: "Publication Date: 2012 Jan 13 PMID: 22244788 Authors: Becker, J. - Wittmann, C. Journal: Curr Opin Biotechnol With a world market of more than four million tons per year, l-amino acids are among the most important products in industrial biotechnology. The recent years have...
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