Engineering tools for Gram positive bacteria
Gram positive bacteria like Bacillus subtilis provide a number of benefits for synthetic biologists. B. subtilis is non-tranformable and naturally transformable, with an efficient system for homologous transformation. Gram positive bacteria provide the bulk of industrially important species and their architectures allow high level secretion of enzymes and other proteins. The Synthetic Biology group at the University of Cambridge is developing tools for work with B. subtilis. The pages in this section provide access to recent papers and relevant websites in the field.
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A growing class of proteins regulates transcription through interaction with DNA-dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase-binding protein that influences the expression of genes under the control of the late-acting, sporulation sigma factor sigmaG in Bacillus subtilis. Spores from a ylyA mutant...
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Both cyanobacteria and eukaryotic microalgae are promising organisms for sustainable production of bulk products such as food, feed, materials, chemicals and fuels. In this review we will summarize the potential and current biotechnological developments. Cyanobacteria are promising host organisms for the production of small molecules that can be secreted such as ethanol, butanol, fatty acids and...
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Aggregates of misfolded proteins are a hallmark of many age-related diseases. Recently, they have been linked to aging of Escherichia coli (E. coli) where protein aggregates accumulate at the old pole region of the aging bacterium. Because of the potential of E. coli as a model organism, elucidating aging and protein aggregation in this bacterium may pave the way to significant advances in our...
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MICROORGANISMS HAVE BEEN COOPERATING WITH EACH OTHER FOR BILLIONS OF YEARS: by sharing resources, communicating with each other, and joining together to form biofilms and other large structures. These cooperative behaviors benefit the colony as a whole; however, they may be costly to the individuals performing them. This raises the question of how such cooperation can arise from natural...
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Publication Date: 2013 Apr 17 PMID: 23599001 Authors: Ma, Q. - Yin, Y. - Schell, M. A. - Zhang, H. - Li, G. - Xu, Y. Journal: Nucleic Acids Res The circular chromosome of Escherichia coli has been suggested to fold into a collection of sequentially consecutive domains, genes in each of which tend to be co-expressed. It has also been suggested that such domains, forming a partition of the genome,...
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Publication Date: 2013 Apr 23 PMID: 23617824 Authors: Obara, B. - Roberts, M. - Armitage, J. - Grau, V. Journal: BMC Bioinformatics BACKGROUND: Microscopy image segmentation lays the foundation for shape analysis, motion tracking, and classification of biological objects. Despite its importance, automated segmentation remains challenging for several widely used non-fluorescence,...
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In 2010 there were more than 200 million cases of malaria, and at least 655,000 deaths. The World Health Organization has recommended artemisinin-based combination therapies (ACTs) for the treatment of uncomplicated malaria caused by the parasite Plasmodium falciparum. Artemisinin is a sesquiterpene endoperoxide with potent antimalarial properties, produced by the plant Artemisia annua....
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P. furiosus Bacterium Missouri University of Science and Technology
To find a way of fending off global warming, scientists sometimes look to nature. Plants, after all, use photosynthesis to snap up carbon dioxide, the biggest source of our climate change woes. So we get inventions like artificial leaves and ambitious projects like a plan to give fish photosynthesizing powers. One of the...
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Publication Date: 2013 Apr 9 PMID: 23572583 Authors: Espinar, L. - Dies, M. - Cagatay, T. - Suel, G. M. - Garcia-Ojalvo, J. Journal: Proc Natl Acad Sci U S A Gene regulatory circuits can receive multiple simultaneous inputs, which can enter the system through different locations. It is thus necessary to establish how these genetic circuits integrate multiple inputs as a function of their...
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Publication Date: 2013 Mar PMID: 23526885 Authors: Perego, M. Journal: PLoS Biol Signal transduction systems are influenced by positive and negative forces resulting in an output reflecting the sum of the opposing forces. The Rap family of regulatory protein modules control the output of two-component signal transduction systems through proteinratioprotein and proteinratiopeptide interactions....
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