Engineering tools for Gram positive bacteria
Gram positive bacteria like Bacillus subtilis provide a number of benefits for synthetic biologists. B. subtilis is non-tranformable and naturally transformable, with an efficient system for homologous transformation. Gram positive bacteria provide the bulk of industrially important species and their architectures allow high level secretion of enzymes and other proteins. The Synthetic Biology group at the University of Cambridge is developing tools for work with B. subtilis. The pages in this section provide access to recent papers and relevant websites in the field.
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Bacterial morphogenesis: learning how cells make cells.: "Publication Date: 2007 Dec PMID: 17703990 Authors: Harold, F. M. Journal: Curr Opin Microbiol Bacteria furnish tractable models for complex biological processes, and morphogenesis is now taking its turn. We can already explain in general terms how such elementary forms as rods and cocci are produced, and the shapes of several...
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Advantages and mechanisms of polarity and cell shape determination in Caulobacter crescentus.: "Publication Date: 2007 Dec PMID: 17997127 Authors: Lawler, M. L. - Brun, Y. V. Journal: Curr Opin Microbiol The tremendous diversity of bacterial cell shapes and the targeting of proteins and macromolecular complexes to specific subcellular sites strongly suggest that cellular organization...
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Thinking about Bacillus subtilis as a multicellular organism.: "Publication Date: 2007 Dec PMID: 17977783 Authors: Aguilar, C. - Vlamakis, H. - Losick, R. - Kolter, R. Journal: Curr Opin Microbiol Initial attempts to use colony morphogenesis as a tool to investigate bacterial multicellularity were limited by the fact that laboratory strains often have lost many of their developmental...
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Shape determination in Bacillus subtilis.: "Publication Date: 2007 Dec PMID: 17981078 Authors: Carballido-Lopez, R. - Formstone, A. Journal: Curr Opin Microbiol The discovery of cytoskeletal elements in prokaryotes has dramatically changed the way we think about bacterial cell morphogenesis. The rod shape of Bacillus subtilis is maintained by the two major polymers (peptidoglycan and...
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Spatial organization of Myxococcus xanthus during fruiting body formation.: "Publication Date: 2007 Dec PMID: 17921303 Authors: Curtis, P. D. - Taylor, R. G. - Welch, R. D. - Shimkets, L. J. Journal: J Bacteriol Microcinematography was used to examine fruiting body development of Myxococcus xanthus. Wild-type cells progress through three distinct phases: a quiescent phase with some...
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Direct selection and phage display of a Gram-positive secretome.: "Publication Date: 2007 Dec 13 PMID: 18078523 Authors: Jankovic, D. - Collett, M. A. - Lubbers, M. W. - Rakonjac, J. Journal: Genome Biol ABSTRACT: Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences by using...
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Bottom-up genome assembly using the Bacillus subtilis genome vector.: "Publication Date: 2007 Dec 9 PMID: 18066072 Authors: Itaya, M. - Fujita, K. - Kuroki, A. - Tsuge, K. Journal: Nat Methods We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B....
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Bacterial development: Moving in the right direction
Nature Reviews Microbiology 6, 6 (2008). doi:10.1038/nrmicro1823 Author: Sheilagh Molloy New data published in a recent issue of Molecular Microbiology might have finally solved the controversy over the function of SpoIIIE during sporulation in Bacillus subtilis.During sporulation, B. subtilis undergoes an asymmetric cell division...
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Recombineering with tolC as a Selectable/Counter-selectable Marker: remodeling the rRNA Operons of Escherichia coli.: "Publication Date: 2007 Dec 15 PMID: 18084036 Authors: Devito, J. A. Journal: Nucleic Acids Res This work describes the novel use of tolC as a selectable/counter-selectable marker for the facile modification of DNA in Escherichia coli. Expression of TolC (an outer...
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Scientists predict extremophile cell's dynamics at genome scale - relevance for biofuels: "
A team of biologists have developed a model mapping the control circuit governing a whole free living organism. This is an important milestone for the new field of systems biology and will allow the researchers to model how the organism adapts over time in response to its environment. By...
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