14 June 2011
Resources for hands-on experiments with Gibson Assembly - a single-tube, isothermal technique for in vitro assembly of up to 10-20 DNA fragments. The technique was developed by Daniel Gibson at the JCVI, as part of the genome assembly effort there. We have found it to be a robust and efficient method for everyday cloning and assembly of DNA-based circuits. In collaboration with the National Centre for Biotechnology Education, and supported by the Synthetic Biology Standards Network in the UK, we are developing scientific and educational resources to promote standardisation and spread of this useful technique. Jim Haseloff and Jim Ajioka, University of Cambridge.
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Monday, September 05, 2011