Publication Date: 2008 Nov PMID: 18794860
Authors: Biteen, J. S. - Thompson, M. A. - Tselentis, N. K. - Bowman, G. R. - Shapiro, L. - Moerner, W. E.
Journal: Nat Methods
The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.