Rapid blue-light-mediated induction of protein interactions in living cells.: "Publication Date: 2010 Dec PMID: 21037589
Authors: Kennedy, M. J. - Hughes, R. M. - Peteya, L. A. - Schwartz, J. W. - Ehlers, M. D. - Tucker, C. L.
Journal: Nat Methods
Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate the utility of this system by inducing protein translocation, transcription and Cre recombinase-mediated DNA recombination using light.
(Via Nature Methods.)