Bottom-up genome assembly using the Bacillus subtilis genome vector

Bottom-up genome assembly using the Bacillus subtilis genome vector: "

Bottom-up genome assembly using the Bacillus subtilis genome vector

Nature Methods 5, 41 (2008). doi:10.1038/nmeth1143

Authors: Mitsuhiro Itaya, Kyoko Fujita, Azusa Kuroki & Kenji Tsuge

We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.