Direct selection and phage display of a Gram-positive secretome.: "Publication Date: 2007 Dec 13 PMID: 18078523
Authors: Jankovic, D. - Collett, M. A. - Lubbers, M. W. - Rakonjac, J.
Journal: Genome Biol
ABSTRACT: Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences by using bioinformatics. However, functional analysis of translated secretomes is possible only if large number of secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins.